Fig. 1

Hippocampal Fndc5/irisin is reduced in mouse VaD models. A Experimental outline. Experimental vascular dementia was established in C57B/6 mice using BCAS. Fndc5 mRNA expression or irisin concentration were respectively assessed using qPCR or ELISA on day 28 after BCAS. B Serum irisin of ageing mice. C Serum irisin of adult mice. D CSF irisin of ageing mice. E CSF irisin of adult mice. F Fndc5 expression and irisin in forebrain of ageing mice. G Fndc5 expression and irisin in hippocampus of ageing mice. H Fndc5 expression and irisin in cerebellum of ageing mice. I Fndc5 expression and irisin in forebrain of adult mice. J Fndc5 expression and irisin in hippocampus of adult mice. K Fndc5 expression and irisin in cerebellum of adult mice. Sham, sham-operated mice; BCAS, mice were subjected to Bilateral common carotid arteries stenosis. (n = 8 per group. All data are expressed as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistical significance)