Fig. 2

BI 113823 treatment reduced BDL-induced liver fibrosis and PH, and improved survival in mice. A Liver HE staining, sirius red staining, α-SMA (red) and B1 receptor (green) IFC stain and their quantification. B Hydroxyproline assay for total collagen in liver; C liver:bodyweight ratio; D bodyweight changes; E changes in portal vein pressure; and F survival rate; G The expression of B1 receptor mRNA in BDL mice livers; H Western blot for the protein expression of B1R, Gly-B2R, B2R, GAPDH in BDL mice livers. Values are mean ± SEM, n = 7–8. p values indicated in panels, ns. not significant and significant as *p < 0.05; **p < 0.01; ***p < 0.001. A–E, G–H One-way ANOVA and Tukey’s multiple comparison test were performed. F Survival curves were analyzed by the nonparametric Mantel-Cox test