Fig. 2

Single-cell RNA-seq profiling revealed cell landscapes derived from atherosclerotic plaques. A UMAP plot of 11,756 scRNA-seq cells, with each color-coded for eight cell types (B cells, Endothelial cells, Mast cells, Monocytes/Macrophages, Neural cells, Plasma cells, T cells, Smooth Muscle cells) (left). Expression levels of representative cell type markers on UMAP feature plots (right). B UMAP plot of expected outcomes of the cell cycle analysis. C Differential expression analysis showing dysregulated genes across each cell type. The adjusted p-value < 0.01 is presented in red, while an adjusted p-value < 0.01 is suggested in black (right). D Communication strength-based and number-based interaction networks showing the ligand-receptor interaction prediction focused on SMC and other GBM cells. Solid lines indicated ligand-receptor pairs, with different subpopulations color-coded (left). The heatmap displaying the specific inferred interactions number and strength of all cells in the atherosclerotic plaque (right). E The circos plots of the ligand-receptor interaction of Collagen and CXCL signaling pathways among cell populations