Fig. 2

Characteristics of tumor cells before and after NACT for gastric cancer. A GSVA of differently expressed pathways in tumor cells before and after NACT. Two-sided unpaired limma moderated t test. B Volcano plot showing DEGs in tumor cells before and after treatment. C t-SNE plots of tumor cell signatures identified by integrative NMF. D Pathway enrichment of three signatures of tumor cells by Metascape. E Comparison of signature score of three tumor cell signatures before and after NACT. ***P < 0.001; n.s.: not significant. F Correlation between tumor cell signatures and prognosis. At the bottom, the number of subjects at risk were showed