Fig. 11

ZBTB9 knockdown blocks the proliferation and metastasis in vitro. The evaluation for efficiency of siRNA via RT-qPCR (A) and WB (B). Colony assay showed the number of ZBTB9 knockdown HepG2 cells was significantly less than siCtrl group cells (C, D). Wound healing assay showed the wound widths of ZBTB9 knockdown groups were significantly wider than the siCtrl group, after 16 h (200 μm) (E, F). With the analysis of the HepG2 cell cycle by flow cytometry, results showed cells in ZBTB9 knockdown groups were significantly stuck in the G1 phase (G, H). Results of the cells transwell assay showed that the migratory cells of ZBTB9 knockdown groups were significantly less than siCtrl groups (100 μm) (I, J). Every experiment was conducted with at least three biological replications (*P < 0.05; **P < 0.01; ***P < 0.001)