Fig. 6

C/EBPβ knockdown inhibited the overexpression of Clec7a and NLRP3 induced pyroptosis in spinal cord of the chronic constriction injury rats. A The double immunofluorescence staining of C/EBPβ and Clec7a in ipsilateral SC of CCI rats after si-Cebpb administration (n = 6 rats/group, 1 section/rat). White arrows point to C/EBPβ- and Clec7a-positive cells. Scale bar: 50 μm. B Representative images (left) and quantification evaluation (right) of Tunel staining in the indicated groups (n = 6 rats/group, 1 section/rat). Red: Tunel-positive cells; blue: nuclei (Hoechst 33,258). Scale bar: 100 μm. Data are presented as mean ± SEM. One-way ANOVA was used to analyze data among multiple groups followed by Tukey’s post hoc test for equal variances or Dunnett T3 post hoc test for unequal variances. ^*P < 0.05, ^**P < 0.01 compared with the Sham group; ^##P < 0.01 compared with sham + si-NC group; ^△P < 0.05, ^△△P < 0.01 compared with CCI + si-NC group