Fig. 2

Protein expression of EMT modulators and activity of G6PD in OVCAR5 cells. A Upregulated of AKR1B1 (35 kDa), ITGAV (125 kDa), TGFβ1 (44 kDa) and G6PD (59 kDa) protein expression was confirmed using western blot. B Densitometry analyses of AKR1B1, ITGAV, TGFβ1 and G6PD were normalized against loading control β-actin (42 kDa). n = 3; mean ± SD; student’s t test; *p < 0.05, **p < 0.01 when compared to OVCAR5 parental cells. C G6PD activity was measured using a colorimetric based assay (MAK015, Sigma Aldrich) according to manufacturer’s instruction. n = 3; mean ± SD; student’s t test; ****p < 0.0001 when compared to OVCAR5 parental cells