Table 1 Primers used in the qRT-PCR assay
Gene | Organisms | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|---|
Gapdh | Mus musculus | GGCAAGTTCAACGGCACAG | CGCCAGTAGACTCCACGACAT |
Col2a1 | Mus musculus | GCTACACTCAAGTCACTGAACAACCA | TCAATCCAGTAGTCTCCGCTCTTCC |
Sox9 | Mus musculus | CGTGGACATCGGTGAACTGAG | GGTGCTGCTGATGCCGTAAC |
P16 | Mus musculus | GGTCACACGACTGGGCGATT | GCACCGTAGTTGAGCAGAAGAG |
P21 | Mus musculus | GCCTGGTTCCTTGCCACTTCTT | ATTACGGTTGAGTCCTAACTGCCATC |
P53 | Mus musculus | CTCCAGCTACCTGAAGACCAAGAAG | GCAGAGACCTGACAACTATCAACCTAT |
Atf3 | Mus musculus | GGTCGCACTGACTTCTGAGG | CTCTGGCCGTTCTCTGGA |
Atf4 | Mus musculus | TGGCGAGTGTAAGGAGCTAGAAA | TCTTCCCCCTTGCCTTACG |
Atf6 | Mus musculus | AGAGGCAGCACACGCATTCC | TGATGGTCAGCAGGAGCAGAGA |
Chop | Mus musculus | CATACACCACCACACCTGAAAG | CCGTTTCCTAGTTCTTCCTTGC |
Ire1α | Mus musculus | CTGGCGAGAAGCAGCAGACTT | CCACCACAGGAGAGGCATAGTT |
Bip | Mus musculus | CCTCATCGGACGCACTTGGAAT | GCTTGTCGCTGGGCATCATTG |
Xbp1s | Mus musculus | GAGTCCGCAGCAGGTG | GTGTCAGAGTCCATGGGA |
Fgf21 | Mus musculus | CAGGGGTCATTCAAATCCTG | AGGAATCCTGCTTGGTCTTG |