Fig. 4

Metabolomics of multivariate statistical analysis on ovarian cancer ascites stages II-IV. A Principal component analysis (PCA) scores plot of OCs. B Heatmap with all the parameters based on stages II-III versus IV. C PCA loadings plot of illustrating features that drive principal component separation. D PCA biplot, green and purple boxes indicate the involvement of in other organs and omentum majus, respectively. E Orthogonal orthogonal partial least-squares discrimination analysis (OPLS-DA) -loadings plot, orange box indicates metabolite involvement in omentum, while red box indicates involvement in other organs. F OPLS-scores plot of OCs illustrates clear II-III and IV group separation