Fig. 6

Granzyme B and perforin co-expressing cells are expanded during Chagas disease. Representative contour plots (A) and frequencies (B) depicting the expression of granzyme B and perforin in MC7 in controls (n = 9) and infected patients with Chagas disease (Chagas, n = 24). C MST shows the differential expression of granzyme B and perforin, represented from blue to red indicating lower to higher expression, respectively. MC7 is highlighted by a red circle. D Representative dot plots of the expression of perforin (x-axis) by granzyme B (y-axis) from controls and infected patients in different stages of Chagas disease (A, B1, B2-C-D). CD4⁺ T cells are colored in gray and each FlowSOM population part of MC7 individually in red. E Contour plots depict the intermediate and high expression of perforin in controls and infected patients in different stages of Chagas disease (A, B1, B2-C-D). F Bars represent the median and interquartile range. Asterisks represent significant differences between the assigned group and controls. *p < 0.05, **p < 0.01, ***p < 0.001