Fig. 5

WZ35 antitumor activity depends upon inhibition of the YAP protein and allows it to regulate the expression of GLUT1. A–C DAPI staining assays A, colony formation assays B and CCK-8 assays C were conducted to measure apoptosis and cellular viability in control or WZ35-treated (10 μg/mL) HCCLM3 cells with or without the pretreatment of knockdown or overexpression via shRNA or pcDNA/peGFP vectors. D Barchart visualized significantly related genes in central carbon metabolism with higher YAP1 expression including 10 genes with the highest logFC and 4 genes with the lowest logFC from datasets GSE97098, GSE77314 and GSE153783. E Correlation between YAP1 and SLC2A1 in liver cancer samples from TIMER dataset (P = 9.94 × 10^–11) was visualized in a scatter diagram. F Western blotting analysis of the YAP and GLUT1 protein level of HCCLM3 cells treated with WZ35 and plasmid. G Real-time qPCR analysis of the YAP and GLUT1 mRNA level of HCCLM3 cells treated with WZ35 and plasmid. H Predicted TEAD binding motif site sequence in the promoter region of GLUT1 host gene chromosome 1 from the database JASPAR