Fig. 2

Adenovirus vector plasmid-mediated knockdown of HNRNPA2B1 in transfected RPMI 8226 cells and corresponding effects on cell proliferation and apoptosis. A Expression efficiency of GFP/RFP fluorescence of adenovirus vector plasmid in transfected RPMI 8226 cells after 72 h. Multiplicity of infection (MOI) refers to the number of virus particles infecting each cell. B qRT-PCR showed HNRNPA2B1 expression was significantly down-regulated at 72 h after transfection of RPMI 8226 cells with the adenoviral vector plasmid. C Western blotting showed HNRNPA2B1 protein expression was significantly down-regulated at 72 h after RPMI 8226 cells with the adenoviral vector plasmid. D CCK8 assay showing reduced proliferation of RPMI 8226 cells with HNRNPA2B1 knockdown compared with control cells. E Flow cytometric detection of apoptosis based on Annexin-V and PI staining in RPMI 8226 cells with HNRNPA2B1 knockdown versus control cells. Diagrams Q-UL, UR, LR, and LL represent necrotic, late apoptotic, early apoptotic, and live cells, respectively. F Statistical results from the apoptosis assay in E