Fig. 5

MICAL1 promoted WNT pathway by phosphorylating TBC1D1. A, B Silver staining (A) and proteomics mass spectrometry analysis (B) of the proteins associated with MICAL1. C Endogenous immunoprecipitation assays were conducted to verify the inosculation between MICAL1 and TBC1D1. D TBC1D1 and phosphorylated TBC1D1 at Ser660 site were detected in indicated PC cells. E, F TOP/FOP flash assays were conducted to evaluate the effect of TBC1D1 silencing (E) or Ser660 site phosphorylation of TBC1D1 (F) on β-catenin-dependent signaling events. H, I Western blot analysis was conducted to evaluate the effect of TBC1D1 silencing (H) or corresponding plasmid transfection (I) on key proteins of WNT pathway