Fig. 4

MICAL1 activated Wnt/β-catenin signaling in PC. A Scatter plot of top 15 enriched KEGG pathways. B MICAL1 stable overexpression cells, MICAL1 stable silencing cells and their respective control cells were transfected with the TOP/FOP Flash reporter plasmids and the luciferase activities were measured 48 h after transfection. C Established downstream target genes of the Wnt/β-catenin pathway, including CCND1, CD44, CMYC, MMP7, TCF4, and TWIST1 were detected by RT-qPCR in indicated cells. D Crucial proteins of WNT pathway including phosphorylation β-catenin at Ser45 site, β-catenin, GSK-3b, AXIN1, APC and intracellular β-catenin were detected by western blotting in indicated cells. Data represent mean ± SD of three independent experiments and were analyzed by two-sided unpaired Student’s t-test, ns = no significance, *P < 0.05, **P < 0.01