Fig. 5

C-myb transcriptionally regulated IL2RA expression by binding to IL2RA. CircLOC101928570 suppresses IL2RA expression. A Prediction of the target gene of the transcription factor c-myb. B The binding sites of c-myb in the promoter of IL2RA are shown in a model. Schematic illustration of the sequences of the wild-type IL2RA promoter domain and mutant sequences in the binding sites of c-myb on the IL2RA promoter domain are shown. C Expression levels of MYB in Jurkat cells after transfection with the MYB plasmid. D Expression levels of MYB in 293 T cells treated with shRNA-NC, shRNA-MYB#1, or shRNA-MYB#2. E, F Relative luciferase activities were detected in 293 T cells after transfecting luciferase reporter plasmids with the wild-type IL2RA promoter domain or the mutant IL2RA promoter domain with pCDH, MYB, shRNA-NC, and shRNA-MYB#1. G, H Western blot analysis of IL2RA proteins in Jurkat cells transfected with circLOC101928570 overexpression plasmid, empty vectors, shRNA-NC, and shRNA-circLOC101928570#1. The results are represented as the mean ± SD (n = 3). NS: no significance, **P < 0.01