Fig. 5
From: Targeted delivery of ZNF416 siRNA-loaded liposomes attenuates experimental pulmonary fibrosis
ZNF416 regulates the activation of fibroblast by promoting the nuclear accumulation of p-Smad2/3. A Protein levels of Smad2/3 and p-Smad2/3 were determined by western blot after ZNF416 overexpression. B, C Western blot analysis of the levels of p-Smad2/3 in the cytoplasm and nuclear after different treatments. D Subcellular localization of p-Smad2/3 in the indicated groups by immunofluorescence assays, p-Smad2/3 stained red, Nuclei were stained by DAPI, scale bar = 25 μm. E, F Coimmunoprecipitation of ZNF416 and Smad2/3 in NIH/3T3 cells