Fig. 2

Genome-wide integration patterns of γ-retroviral and lentiviral vectors.A The percentage of viral integration sites on each human chromosome in CAR T-cells produced with lentiviral and γ-retroviral vectors is shown. B A scheme of expected integration sites in different genomic features. The viral genome is labeled as red rectangles. C The bar chart depicts the percentage of integration sites found in the intron, exon, promoter, untranslated region (utr, which includes 3’utr and 5’utr), and not in the gene region in all products. The x-axis represents mean coverage percentage (%). The y-axis represents each genomic feature. The number under each genomic feature in y-axis represents the mean coverage number of all samples in γ-retroviral vector group and lentiviral vector group. Data in boxplot were analyzed by using two-tailed unpaired Student’s t-Test. ***p < 0.001. D Representative patterns from 4 different CAR T-cell products showing the relative position of viral integration from the transcription start site (TSS). #053 CD22-CAR and #064 CD19/22-CAR were made with lentiviral vectors. #056 BCMA-CAR and #069 SLAMF7-CAR were made with γ-retroviral vectors. The x-axis indicates the relative position from transcription start site. The y-axis represents normalized signal