Fig. 2

Characterization of exosomes and the effects of radiation on exosomes phagocytosis by KYSE150 and ECA109. A The expression of HMGB1 in ESCC cell lines. B Transmission electron micrograph of Exos and IR-Exos. C The size distribution and the concentration of the exosomes were measured with Nanoparticle Tracking Analysis. D Western blotting of exosomes and cell lysate proteins was performed to confirm exosome marker proteins (CD9, TSG101, HSP70) and calnexin. E, F Changes of HMGB1 protein in KYSE150 and ECA09 after GW4869. G DIL (red)-labeled exosomes were co-cultured with KYSE150 and ECA109 for 24 h. Then the phagocytosis of exosomes and the HMGB1(green) position changes before and after irradiation were observed (DAPI-labeled nuclei were blue, magnification, ×600). *P < 0.05; **P < 0.01; IR: irradiation; HMGB1: high mobility group box 1; Exos: exosomes