Fig. 2

High expression of ACE2 is associated with drug resistance in breast cancer patients. A The ACE2 expression profiles of breast cancer samples and normal tissues in the TCGA-BRCA and GTEx database. (Dot plot) Each dot represents the expression of a sample (*P < 0.05). B Western blot analysis showed the expression of ACE2 protein in normal breast cell line (MCF-10A) and breast cancer cell lines (MDA-468, MDA-231, T47D, MCF-7, BT-549, SK-BR-3, BT-474). The number below every lane represents the ratio of ACE2/β-actin expression in the Western blot assays. C Expression levels of ACE2 gene in breast cancer, colon colorectal cancer, lung cancer, kidney cancer and pancreatic cancer cell lines from the Cancer Cell Line Encyclopedia (CCLE). The vertical axis in the figure represents ACE2 expression, the horizontal axis is the cell lines, and the different colors represent the group of cell lines. D ACE2 expression levels in the plasma of all breast cancer patients (n = 111) and healthy controls (n = 20) were measured by ELISA (P = 4.7e−12). E The plasma levels of ACE2 in chemosensitive and insensitive breast cancer patients (P = 3.9e-06). (F) Comparison of ACE2 expression levels in breast cancer patients receiving and not receiving chemotherapy in METABRIC (***P < 0.001) and (G) TCGA-BRCA database (****P < 0.0001)