Fig. 3

Lymphocytes stimulated by DCs pulsed with DWCTVs in vitro. a Flow chart of experimental plan adopted for the in vitro lymphocyte stimulation assays. b, c The CD4/CD8 ratio of CD3 + cells. d, e IFNG mRNA expression level of lymphocytes. The IFNG mRNA expression level of lymphocytes stimulated by imDC and DCs pulsed with A2780 and OVCAR3 DWCTVs was detected by RT-qPCR. The relative expression level was normalized to the GAPDH expression level and calculated by 2^−ΔCT × 1000. f, g DWCTVs enhance DC-mediated cytotoxicity of T cells in vitro. The killing activity of corresponding T cells (effector cells) to parental OC cells (target cells) shown are from the effector/target ratio at 1:1, 5:1, and 10:1. Unmodified, α2-3NA and α2-6NA represent the stimulation of DCs pulsed with unmodified WCTV, α2-3NA modified DWCTV and α2-6NA modified DWCTV, respectively. Data are presented as mean ± SD (n = 3). The statistical test of b–e used is an unpaired t-test, while it is paired t-test for (f–g). *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0001