Fig. 6

The over-expression ofAnxa2reversed the inhibitory effects of p-STAT3 on hepatocyte pyroptosis and fibrosis in NASH mice. (A) HE staining for indicating the pathological changes; Masson staining for detecting liver fibrosis of mice; Immunohistochemical staining for detecting α-SMA expression. (B) WB for detecting the expressions of p-STAT3, ANXA2, and pyroptosis-related proteins (ASC, pro-Caspase1, C-Caspase1, GSDMD, and GSDMD-N) in the liver tissues of mice in each group. (C) Differentially analysis of the α-SMA positive area. (D) The serum levels of ALT, AST, IL-1β, and CRP. Eight mice were included in each group. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001