Fig. 2

TCF7 promotes the proliferation and fibrosis of HLF cells in vitro. A The transfection effect of TCF7 overexpression or knockdown in HLF cells was confirmed by RT-qPCR and Western Blot. B The effect of overexpression or knockdown of TCF7 on the proliferation of HLF cells was detected by CCK-8 assay. C, D The effect of overexpression or knockdown of TCF7 on the apoptosis of HLF cells was assessed by flow cytometry assay. E The influence of overexpression or knockdown of TCF7 on the protein expression of apoptosis-related genes (cleaved caspase3, Bax, and Bcl-2) in HLF cells was confirmed by Western Blot. F The influence of overexpression or knockdown of TCF7 on the protein expression of fibrosis-related genes (collagen I, collagen III, MMP2, MMP13, and TGFβ1) in HLF cells was confirmed by Western Blot. C-casp3, cleaved-caspase3; col.I, collagen I; col.III, collagen III. shNC, corresponding negative control of TCF7 knockdown adenovirus; shTCF7, TCF7 knockdown adenovirus; mock, corresponding negative control of TCF7 overexpressed adenovirus TCF7; TCF7 overexpressed adenovirus. ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001