Fig. 7

Validation of the orchestrated roles of TAM1 and C4 cells. A. Multiplex IHC designed for the macrophage biomarker panel and fibroblast panel. B. Representative multicolor IHC staining for CD68⁺CD163⁺ IDO1⁻ S100A8⁻ TAM1, CD68⁺CD163⁻ IDO1⁺ S100A8⁻TAM2 and CD68⁺CD163⁻ IDO1⁻ S100A8⁺ TAM3. C. Representative multicolor IHC staining of VIM⁺ FAP⁺ WT1⁻S5 and VIM⁺FAP⁺WT1⁺S6 cells. D. Dot plot of the correlation between the infiltration level of TAM1-3 with the surrogate score of C4 cells in tissue arrays 1 and 3. Each dot represents a CRC patient sample. Correlation test is determined using Pearson test. E. Overall survival curves for TAM1-3 in tissue arrays 1 and 2 with sample size of each group placed on the bottom. HR, 95%CI and p value are annotated in text