Fig. 6

ADM knockdown partially attenuated the effects of miR-1297 inhibition on TMZ-treated glioma cells LN-229 cells were co-transfected with sh-ADM and miR-1297 inhibitor, treated with TMZ, and examined for miR-1297 expression by RT-PCR (A); cell viability by CCK-8 assay (B); ADM expression by immunoblotting (C); cell apoptosis by Flow cytometry (D); the protein levels of cleaved caspase 3, pro-caspase 3, cleaved caspase 9, pro-caspase 9, Bax, Bcl-2, p-Akt, Akt, p-Erk1/2, and Erk1/2 by Immunoblotting (E); mitochondrial membrane potential changes by JC-1 staining (F)