Fig. 2

ADM knockdown sensitizes glioma cells to TMZ treatment. A ADM knockdown was achieved in LN-229 cells by transfecting short hairpin RNA targeting ADM (sh1-ADM or sh2-ADM); ADM knockdown was confirmed using qRT-PCR. Then, LN-229 cells were transfected with sh-NC or sh-ADM, treated or non-treated with TMZ, and examined for cell viability by CCK-8 assay (B); cell apoptosis by Flow cytometry (C); the protein levels of ADM, cleaved caspase 3, pro-caspase 3, cleaved caspase 9, pro-caspase 9, Bax, Bcl-2, p-Akt, Akt, p-Erk1/2, Erk1/2 and cleaved PARP by Immunoblotting (D); mitochondrial membrane potential changes by JC-1 staining (E)