Fig. 6

SIRT3 participated in the regulation effect of omentin1 on unbalanced mitochondrial fusion-fission dynamics and mitophagy. A Representative images of western blotting analysis of the expression of FOXO3a in cardiomyocytes treated with omentin1 and 3-TYP (n = 3). B Representative images of immunofluorescent analysis of the expression of FOXO3a in nucleus of cardiomyocytes treated with omentin1 and 3-TYP and the statistical results using quantitative analysis of Pearson’s correlation coefficient were presented, scale bar = 5 μm (n = 3). C Representative images of mitochondrial membrane potential (ΔψM) were presented, scale bar = 20 μm. Green fluorescence represents the dissipation of ΔψM. Red fluorescence represents intact ΔψM (n = 3). D Representative images of mitochondrial SOX were presented, which represents the generation of mitochondria-derived ROS, scale bar = 20 μm (n = 3). Representative images of western blotting analysis of the expression of E Mfn2, F L-OPA1/S-OPA1 and G p-Drp1(Ser616)/Drp1 in mitochondria of cardiomyocytes treated with omentin1 and 3-TYP (n = 3). Representative images of western blotting analysis of the expression of H Parkin, I PINK1 and J p62 in mitochondria of cardiomyocytes treated with omentin1 and 3-TYP (n = 3). Results were gained from three independent experiments and were expressed as mean ± SD. *P < 0.05, ***P < 0.001 vs. the OGD group; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, ^$$$$P < 0.0001 vs. the OGD + omentin1 group. A–J one-way ANOVA