Fig. 4

XPO1 inhibitor KPT-330 induced GBC cells apoptosis by reducing mitochondrial membrane potential. A Fluorescence images of TUNEL in NOZ and GBC-SD showed increased green fluorescence after KPT-330 treatment relative to the DMSO treatment group. Scale bars represent 100 μm. B Flow cytometry using PI/Annexin V-FITC double staining showed KPT-330 induced apoptosis of NOZ and GBC-SD. C and D Expression of BAX, Bcl-2, Cleaved PARP/PARP, Cleaved caspase 9/caspase 9, Cleaved caspase 3/caspase 3 was detected by western blot. E Flow cytometry analysis by using JC-1 staining. The horizontal axis channel “FL1-H” is the same as the FITC channel; the vertical axis channel “FL2-H” is the same as the PI channel. Student's t test was applied to the statistical analysis in this figure. Data presented as mean ± SD (n = 3)