Fig. 2

MIR99AHG targets miR-136-5p to exert its tumor suppressive effects in LUAD. A DIANA database analysis of LncRNA MIR99AHG binding sites to miR-136-5p. B Expression of MIR99AHG was examined by RT-qPCR after knocking down miR-136-5p. C Expression of miR-136-5p was examined by RT-qPCR after overexpression of MIR99AHG. D Putative binding sequence between MIR99AHG and miR-136-5p. E After transfection in HEK293T cells according to experimental subgroups, luciferase activity analysis (n = 3). F Luciferase reporters containing MIR99AHG-Luc-WT were co-transfected with or without miR-136-5p and MIR99AHG, Luciferase activity was determined using a dual luciferase reporter system. G FISH results show the colocalization of MIR99AHG and miR-136-5p in the cytoplasm of LUAD cells