Fig. 6

Rab27a facilitates THLE-3 cell malignant phenotypes via increasing the exosome secretion of VEGF and TGF-β. A Rab27a expression in THLE-3 cells was analyzed after Rab27a overexpression. N = 3. B, C The proliferation of THLE-3 cells was assessed via EdU and colony formation assays after Rab27a overexpression. N = 3. D, E Cell migratory and invasive abilities were evaluated through wound healing and transwell assays after Rab27a overexpression. N = 3. F, G The EMT process of THLE-3 cells was evaluated by western blot and immunofluorescence staining after Rab27a overexpression. N = 3. Scale bar = 50 µm. H, I The protein concentration of VEGF and TGF-β in THLE-3 cells was analyzed via RT-qPCR and western blot analyses with Rab27a overexpression. N = 3. *P < 0.05, **P < 0.01