Fig. 2

Overexpression of 11β-HSD1 elevated neuronal excitability in vitro. A, B Western blot images showing 11β-HSD1 expression in the primary cultured neurons transfected with 11β-HSD1 overexpression plasmid (11β-HSD1+/+) or control plasmid. C Raw trace of an action potential at depolarization recorded under current clamp mode. The lower panel shows an expanded image of the same action potential, with a dotted line demonstrating the action potential threshold. D Representative images of the APs recorded by patch clamp technique. E, F Statistical analysis of the threshold voltage and frequency of APs (*P < 0.01, compared to control group). The bars indicated the mean ± SD