Fig. 8

Effect of IL13RA1 knockdown on the expression of CAD-relevant factors. A. Significant difference in protein expression between BMDMs treated by PBS or ox-LDL. B. Relative statistical results of the protein bands in A(P < 0.05). C. Difference in protein expression between CAD model and negative control group mice aortas. D. Relative statistical results of the protein bands in C. E. Relative mRNA expression of IL13RA1 in BMDMs treated by PBS or ox-LDL. F. Relative mRNA expression of IL13RA1 in CAD model/negative-control group mice aortas. G. Reddish fluorescence indicating the transfection efficiency of Cy3-labeled scrambled siRNA. H. mRNA expression of IL13RA1 in RAW264.7 after transfected with siRNA. I. Difference in proteins expression among si-negative control(si-nc), si-IL13RA1(si-IL13RA1), si-nc with ox-LDL (si-nc + ox-LDL) and si-IL13RA1 with ox-LDL (si-IL13RA1 + ox-LDL) groups. J. Relative statistical results of the protein bands in I. K. Oil red O staining of ox-LDL-stimulated RAW264.7 cells with/without IL13RA1 knockdown. L. Relative statistical results of the stained area in K. M. Relative mRNA expression of SCRAB1 among si-nc, si-IL13RA1, si-nc + ox-LDL and si-IL13RA1 + ox-LDL groups. N. Relative mRNA expression of CD36 among si-nc, si-IL13RA1, si-nc + ox-LDL and si-IL13RA1 + ox-LDL groups. Si-nc vs. si-IL13RA1, *P < 0.05, **P < 0.01, ****P < 0.0001. Si-nc + ox-LDL vs. si-IL13RA1 + ox-LDL, #P < 0.05, ##P < 0.01, ###P < 0.0001