Fig. 3

Volcano plots of small RNA samples. The figure shows volcano plots comparing log₂fold-changes of read counts (x-axis) to -log₁₀ of the corresponding adjusted p-values (y-axis) for all small RNA read counts in each comparison. The colors of the dots indicate for the respective small RNA whether the number of read counts exceeds a log₂fold-change threshold of smaller than − 2 or larger than 2, respectively, represented by the dashed vertical lines, and whether the adjusted p-value of the − log₁₀ is larger than 1,30103 (horizontal dashed line, p ≤ 0,05). Grey dots represent read counts of small RNAs with adjusted p-value > 0.05 and a log₂-change ≤ 2 and ≥ -2 (not significant), green dots represent read counts with log₂fold-changes of ≤ -2 and an adjusted p-value of p ≤ 0.05, blue dots show read counts with log₂fold-change of ≤ 2 and ≥ -2 but an adjusted p-value of p ≤ 0,05, red dots display read counts with log₂fold-change ≥ 2 and an adjusted p-value of p ≤ 0,05, yellow dots display read counts with a log₂fold change ≤ -2 or ≥ 2 but an adjusted p-value of p > 0,05 (not significant). Figure A shows the comparison of CSF EV versus whole CSF, figure B compares serum EV and whole serum, figure C serum EV and whole CSF, figure D serum EV and CSF EV, figure E whole serum and CSF EV, and figure F whole serum versus whole CSF. Altogether 4601 variables were analyzed in each plot