Fig. 1

Generation and characterization of iPSC-derived NPCs. A: Schematic representation of the analyzed cell types, time frame and the main marker proteins used for characterization. B: Principal component analysis of qPCR data for NPCs (3 clones, dark blue), iPSCs (3 clones, light blue), NPCs 21d after neural differentiation (clone 1, violet) and human total brain RNA. Each symbol illustrates data from RNA extracted from one cell culture well. C: Gene expression of pluripotency marker (NANOG and OCT4) and NPC marker (PAX6, SOX1, CXCR4, MSI1, NESTIN) in NPCs over the course of 15 passages, measured by qPCR. D: iPSCs (left panel) and NPCs at passage 7–10 (right panel) stained for Pax6, Nestin, Oct4 and DAPI. E: Flow cytometry analysis of iPSCs (upper row) and NPCs at different passages (lower rows) for Oct4, Pax6 and Sox1. Pie charts illustrate percentage positivity (light/dark blue) for the respective marker and cell type. n.t.: not tested Neural diff: NPCs after neural differentiation; CXCR4: CXC-motif chemokine receptor-4; MSI1: Musashi-1; Scale bars: 50 µm. Significance of mean differences between the groups was assessed using Tukey’s HSD. Asterisks indicate significance: *** P < 0.001