Fig. 5

Poised chromatin and miR-32 decreased the expression of MARCH8 in CRC. A ChIP-Seq binding peaks in the MARCH8 gene promoter of histone modification marks; B MiRwalk, TargetScan and miRDB were applied to predict the binding sites of miRNAs in the MARCH8 3’UTR; C Expression correlation analysis of miR-199b and miR-32 with MARCH8 in TCGA COAD and READ datasets; D Expression of MARCH8 in colorectal cells was examined with qRT–PCR in miR-32 inhibitor-treated cells; E A luciferase assay was conducted to explore the binding sites of miR-32 with MARCH8 3’UTR sites. F Schematic figure showing the molecular mechanism of the miR-32/MARCH8/HK2 axis in CRC. *P < 0.05, **P < 0.01 vs. Vector