Fig. 7

HO1 downregulates CD48 through modulation of Sirt1. A Proteins from THP-1 cells transduced with empty vector and HO1 were isolated and analyzed by western blotting using antibodies against acetyllysine or di-methyllysine. B Network diagrams for HDACs association studies related to HO1 were generated using GCBI online tool. C qRT-PCR assay of Sirt1 gene levels in HO1 high/low expression groups (n = 40). D Correlation between HO1 and Sirt1 expression in AML samples as evaluated by qRT-PCR. E HDAC4, HDAC8, and Sirt1 protein levels in AML samples as determined by western blotting (n = 10). F Sirt1 mRNA and protein expression levels in leukemia cell lines as evaluated by qRT-PCR and western blotting. G Left graph: flow cytometry analysis of CD48 expression in THP-1 cells treated with DMSO (gray line) or with SRT1720 (red line). Right graph: flow cytometry analysis of CD48 expression in MV4-11 cells treated with DMSO (gray line) or with selisistat (blue line). H The mRNA and protein level of Sirt1 was detected in THP-1 cells overexpressed HO1 and MV4-11 cells silenced HO1. I Left graph: flow cytometry evaluation of CD48 levels in THP-1 cells transduced with LV-HO1 (red line) or with an empty vector (gray line). LV-HO1 cells treated with selisistat (blue line) are also shown in this graph. Right graph: flow cytometry evaluation of CD48 levels in MV4-11 cells transduced with Si-HO1 (red line) or an empty vector (gray line). Si-HO1 cells treated with SRT1720 (blue line) are also shown in this graph. Statistical differences were determined using the Student’s t-test. ns, no significance