Fig. 3

Enhanced inhibitory function of Tregs from DLBCL patients. A Cytotoxic activity of activated CD8⁺CTLs cocultured with or without Tregs from DLBCL patients (D-Treg) and healthy donors (N-Treg) toward CFSE-labelled SU-DHL4 cells at the E/T ratios of 10:1. The percentage of PI⁺ cells were used to calculate the % lysis of target cells by CD8⁺ CTLs. n = 3. B PI staining of SU-DHL4 cells from the co-culture system of CD8⁺CTLs with Tregs from DLBCL patients (D-Treg) and healthy donors (N-Treg), or without Tregs at the E/T ratios of 5:1,10:1 and 20:1. n = 3. C, D Proliferation of CD8⁺ CTLs was determined by coculturing CD8⁺ CTLs with or without Tregs from DLBCL patients (D-Treg) and healthy donors (N-Treg) for 3 days. The percentage of Ki67⁺ cells was used to calculate proliferation of CD8⁺ CTLs. n = 3. E, F Intracellular expression of GzmB in CD8⁺ CTLs cocultured either alone or with Tregs from DLBCL patients (D-Treg) and healthy donors (N-Treg) for 3 days. n = 3. G, H CD107a surface expression on CD8⁺ CTLs exposed to SU-DHL4 cells alone or in the presence of Treg cells from DLBCL patients (D-Treg) and healthy donors (N-Treg) for 4-5 h. n = 3. *P < 0.05; **P < 0.01, ***P < 0.001. Asterisk indicates significant difference from control group as assessed. Pound sign indicates significant difference from different Treg group. p values were determined by one-way ANOVA; data are presented as mean values ± SEM