Fig. 1

Identifying and characterizing circCRKL in BCR-ABL⁺ cell lines and CML samples. A Differential expression of circCRKL between CML and normal donors samples. B Relative circCRKL expression in a normal lymphoblastic cell line (TK6) and BCR-ABL⁻ leukemia cell line (THP-1) and BCR-ABL⁺ leukemia cell lines (K562, K562/G01, KCL22, and SupB15). C Schematic diagram of circularization of circCRKL, the back-splice site was identified using sanger sequencing. The cytoplasmic distribution of circCRKL in K562 cells (D) and K562/G01 cells (E). F FISH assay demonstrated that circCRKL was predominantly expressed in cytoplasm in K562 and K562/G01 cells (Cy3 was used for labeling circCRKL probe). G, H After treating K562 and K562/G01 cells with or without RNase R, the relative expression of circCRKL and linear CRKL was measured. I, J Relative expression of circCRKL and linear CRKL following treatment with actinomycin D in K562 and K562/G01 cells. *p < 0.05, **p < 0.01, ***p < 0.001 and **** < 0.0001