Fig. 2

Age, smoking history, and cancer type affect the TML in all patients. (A) TML by age-range. > 60 years old, n = 713, ≦ 60 years old, n = 208. (B) TML by smoking history. Lifelong non-smoker, n = 83, ≦ 30 cigarette packs smoking, n = 235, > 30cigarrette packs, n = 491. (C, D) Statistically meaningful differences between the median of the TML distributions were found only for LUAD patients, hinting to LSCC having other origins than smoking. Current smokers have the highest TML for both age groups, and, within that group, young patients seem to have a higher TML than older patients. LUAD: lung adenocarcinoma; LSCC: lung squamous cell carcinoma. TML: Total Mutational Load; ***** p < 0.0001; ** p < 0.001; NS non-significant