Fig. 4
From: Gene expression and methylation profiles show the involvement of POMC in primary hyperparathyroidsm
Expression of POMC in the PHPT and normal cohorts. A POMC mRNA expression between 3 normal parathyroid samples (N05–N07) and 3 parathyroid adenoma samples (P07–P09) by qRT-PCR. P = 0.0086. B POMC protein expression in the normal and PHPT cohorts by immunohistochemistry. Paraffin-embedded tissues from 11 normal parathyroid samples (N11–N21) and 31 parathyroid adenomas (P13–P43) were analyzed. P = 0.0005. C POMC protein expression in normal parathyroid tissue (N08–N10) and PHPT parathyroid tissue (P10–P12) by western blot and was shown as histogram (D). P = 0.0002. E The methylation levels of the POMC promoter region in normal parathyroid tissue (F) and parathyroid adenoma (G). There were 16 samples for the experiment, including 6 normal parathyroid tissues (N16–N21) and 10 PHPT parathyroid tissues (P23–P32). Each experiment was done in triplicate. M represents the amplification products with the methylated primers. U indicates the amplification products with the unmethylated primers. POMC expression in normal parathyroid glands (H, I, J, K) and parathyroid adenoma (L, M, N, O) via immunohistochemistry. The right series of microphotographs (I, K, M, O) were taken from the parts shown in the white boxes in the left microphotographs (H, J, L, N). Hematoxylin and eosin staining of normal parathyroid glands (P) and parathyroid adenoma (Q). Scale bar: 50 um.