Fig. 3

GAS6-AS1 promotes CRC tumorigenesis and metastasis in vivo. A Representative images and the volume of tumors derived from HT29 cells with or without GAS6-AS1 overexpression. B Representative images and the volume of tumors derived from LoVo cells with or without GAS6-AS1 inhibition. C, D The weight of formed tumors. E HT29 cells with or without GAS6-AS1 overexpression were injected into the tail vein of mice to construct lung metastasis models, the representative images and numbers of metastatic nodules in the lungs. F LoVo cells with or without GAS6-AS1 knockdown were injected into the tail vein of mice to construct lung metastasis models, the representative images and numbers of metastatic nodules in the lungs. The tumor micro metastasis nodules in the lungs were counted according to H&E staining. (scale bar: 50 μm). ** P < 0.01, ** P < 0.01, *** P < 0.001