Fig. 2

ERRα was essential for metabolic reprogramming in Dox-resistant OS cells. The relative levels of ATP generation (a), consumption of glucose (b), and production of lactate (c) in MG-63/Dox and HOS/Dox cells treated with XCT-790 (1 μM) or vehicle control for 24 h; the variation of OCR in MG-63/Dox (d) and HOS/Dox (e) cells treated with XCT-790 (1 μM) or vehicle control for 24 h was determined by extracellular flux analysis. The variation of ECAR in MG-63/Dox (f) and HOS/Dox (g) cells treated with XCT-790 (1 μM) or vehicle control for 24 h was determined by extracellular flux analysis. h The protein expression of ERRα in MG-63/Dox cells transfected with si-NC or si- ERRα-1/-2 for 24 h were measured by western blot analysis (left) and quantitively analyzed (right); MG-63/Dox cells were transfected with si-NC or si-ERRα for 24 h, and then the relative levels of ATP generation (i), consumption of glucose (j), and production of lactate (k) were measured. Data are presented as means ± SD from three independent experiments. Each experiment was performed in six replicates for OCR or ECAR analysis. ^**p < 0.01 by Student’s t test