Fig. 5
From: Harm of circadian misalignment to the hearts of the adolescent wistar rats
A The Flameng score of myocardial mitochondria measured using a fluoroscopy electron fiberscope. Expression of CD34 (B), HSP60 (C), CACNA1C (D) and BECLIN-1 (E) in the two groups of rats measured by immunofluorescence staining. *p < 0.05, **p < 0.01, ***p < 0.001 (two-sample t-test). (F) Transmission electron microscope scanning images of the myocardial mitochondria from the two groups of rats. A large number of myocardial mitochondria in the CM group showed vacuolar changes and cristae fractures, indicating a significant increase in ruptured mitochondria. Immunofluorescence staining for CD34 (G), HSP60 (H), CACNA1C (I) and BECLIN-1 (J). Blue represents the nucleus stained with DAPI, and red represents positive expression. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; HSP60, heat shock protein 60; CACNA1C, Calcium Voltage-Gated Channel Subunit Alpha1 C; BECLIN-1, Coiled-Coil, Moesin-Like BCL2-Interacting Protein