Fig. 3

BET inhibitors treatment suppressed EC cell growth. A EC cells were treated with 0–10 µM JQ1 for 1–5 days. The cell vaibility of JQ1 was measured using MTT assay. B EC cells were treated with DMSO or 0.1 µM JQ1 for two weeks. The growth inhibition effects of JQ1 were measured using cell colony formation assay in four EC cell lines. C The relative expression levels of BRD4 were measured by western blot analysis in four EC cell lines. β-Actin served as the loading control. D EC cells were treated with 0–40 µM OTX015 or I-BET151 for 2 or 4 days. The growth inhibition was measured using MTT assay. E EC cells were treated with DMSO or 0.1 µM OTX015 (left side) or 0.5 µM I-BET151 (right side) for two weeks. The growth inhibition effects were measured using cell colony formation assay in Ishikawa and HEC-1A cell lines. Data shown are mean ± SD from three independent experiments