Fig. 2
Appraisal of VEGFB gene knockout mice and NAFLD cell model with low expression of VEGFB. A VEGFB gene identification in wild-type and homozygous-type mice. B Relative mRNA expression level of VEGFB gene in heart and muscle tissue. C The content of VEGFB was detected by Elisa in the plasma of VEGFB systemic knockout wild-type and homozygous-type mice. D iWAT、eWAT and liver tissue of VEGFB gene knockout mice were analyzed by Western Blot. E VEGFB Protein expression level in iWAT、eWAT and liver tissue of VEGFB gene knockout mice. F–H Analyses of relative mRNA level of iWAT, eWAT and liver tissue in 3 groups of mice by RT-PCR. I HepG2 cell viability was induced by different concentrations of PA. J, K Levels of TG and TCHO in HepG2 induced by PA. L Western blot test of VEGFB in HepG2 cells after siRNA transfection. M Expression level of VEGFB protein in NAFLD cell model. N Relative mRNA expression level of VEGFB gene in NAFLD cell model. N = 6 cases per group. A–C * indicates vs. VEGFB+/+. D–H * indicates vs. SD, p < 0.05; # indicates vs. HFD, p < 0.05. I–N * indicates vs. NC, p < 0.05