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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Interplay of Helicobacter pylori, fibroblasts, and cancer cells induces fibroblast activation and serpin E1 expression by cancer cells to promote gastric tumorigenesis

Fig. 1

Identification of CAFs and NFs. A Morphology of CAFs and NFs. Scale bar = 50 μm. B Immunofluorescence staining for α-SMA, FAP, FSP-1, and Vimentin in CAFs and NFs. Vimentin, a specific marker of stroma cells, was used as a control. Scale bar = 25 μm. C Western blot analysis shows the higher expression of α-SMA and FAP in CAFs than in NFs. D Growth curve of CAFs and NFs was performed with CCK-8 assay at indicated time points. Each plotted data point represents the mean ± SD (n = 6). *P < 0.05. E Colony formation of AGS cells co-cultured with CAFs or NFs for ten days at CAFs/NFs: AGS cells ratio of 10:1 (upper panel) and the morphology of the co-cultured AGS cells and CAFs under a light microscope (middle panel). The bar graph shows the quantification of colony number (lower panel). The data are shown as mean ± SD (n = 3). Scale bar = 100 μm. *P < 0.05

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Journal of Translational Medicine

ISSN: 1479-5876

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