Fig. 1

Phenotypic characterization and immunomodulatory effects of rCPCs. Rat CPCs were prepared by sorting for rat CD117 of cells prepared from the right atrial appendage of the heart of Wister Kyoto. A Rat CPCs and rat MSCs characterized for expression of the CD117, CD105, CD90, CD45 and CD31 markers. The expression level of RT1A, RT1D, CD80 and CD86 were measured for rCPCs and compared with rMSCs. B In vitro T cell proliferation in combinations of syngenic, allogenic and xenogenic cells. C–E Results of cytokine analysis by ELISA of syngenic and allogenic and xenogenic combinations. Percentage of T-regulatory cells (T-regs) detected by incubating rCPCs for 5 days with CD4⁺ T cells isolated from WK and BN spleens in vitro. F, G Show T-reg for syngenic and H, I illustrate T-reg in allogenic combinations. Numerical data are summarized as box and whisker plots with a median value (black bar inside box), 25th and 75th percentiles (bottom and top of box, respectively), and minimum and maximum values (bottom and top whisker, respectively). The number (n) of rats in each group indicated near the (up/below/on) each respective box and whisker plot