Fig. 1

Expression level of SEMA4D in AML. A Expression level of SEMA4D in LAML compared with healthy control in GEPIA Database. B The OS of patients with LAML in GEPIA Database. C qRT-PCR was used to detect SEMA4D mRNA level in BM-MNCs of AML patients and healthy donors. D Western blot was used to detect SEMA4D protein levels in BM-MNCs of AML patients and healthy donors. E qRT-PCR was used to detect SEMA4D mRNA level in U937 and Molm-13. F Western blot was used to detect SEMA4D protein levels in U937 and Molm-13. Results of densitometry analysis of relative expression levels after normalization to loading control β-actin are presented. G Expression of SEMA4D in LAML based on risk status in TCGA database. Data with statistical significance are as indicated, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant