Fig. 5

Co-culture with MSCs up-regulated autophagy markers in AML cells with FLT3-ITD mutation. As compared with that without MSCs co-culture, MSCs, no matter from the relapsed patient (case #3, A) or from the CCR case (case #2, B) with FLT3-ITD mutation, significantly increased the number of phagosomes (under the arrows) in MOLM14 cells being detected with transmission electron microscopy. Immunoblotting analyse showed MSCs, from the relapsed patient (case #3, C) or from the CCR case (case #2, D) with FLT3-ITD mutation, increased the expression of LC3B-II, Beclin-1 and ATG5, and degraded p62 expression in MOLM14 cells