Fig. 5
In vitro studies revealed that overexpression of HHLA could significantly increase the cellular migration abilities by using wound-healing assay and transwell assay. A and B The wound-healing assay revealed that in 786-O cells, after depletion of METTL3, overexpression of HHLA2 could significantly increase the cellular migration abilities (LV-METTL3-shRNA v.s. LV-METTL3-shRNA + LV-HHLA2OE, P < 0.01). C and D The wound-healing assay revealed that in ACHN cells, after depletion of METTL3, overexpression of HHLA2 could significantly increase the cellular migration abilities (LV-METTL3-shRNA v.s. LV-METTL3-shRNA + LV-HHLA2OE, P < 0.01). E and F The transwell assay results confirmed that, in both 786-O and ACHN cells, overexpression of HHLA2 could significantly increase the cellular invasion abilities (LV-METTL3-shRNA v.s. LV-METTL3-shRNA + LV-HHLA2OE, P < 0.01 respectively). Un-paired t test was used as needed. **P < 0.01, ***P < 0.001