Fig. 2

CircMID1 knockdown inhibits cell proliferation, invasion, and migration in MDSC-Exo-treated PC3 cells. A Relative expression of circMID1 in PC3 cells transfected with specific siRNAs against circMID1 (^**p < 0.01). B CCK-8 assays were used to measure cell proliferation in control PC3 cells and MDSC-Exo-PC3 cells transfected with si-control or si-circMID1 (^**P < 0.01). C, D Transwell assays were used to determine cell migration (C) and invasion (D) in control PC3 cells and MDSC-Exo-PC3 cells transfected with si-control or si-circMID1. Scale bar, 100 μM. ^*, ^#P < 0.05; ^**, ^##P < 0.01, ^* vs. control, ^# vs. Exo. All the experiments were repeated at least three times